additional studies

Researchers can submit an application for an additional study. This allows them to obtain additional questionnaires or samples from a group of Lifelines participants. 

additional studies

The Lifelines cohort study has an open protocol. On the one hand, this means that a fixed protocol is observed to follow study participants over time. On the other hand, it offers researchers the opportunity to collect additional data and/or biological samples within the existing infrastructure. To this end, researchers can submit an application for an additional study. This allows them to obtain an additional questionnaire or collect additional samples among a group of participants. Please contact Lifelines for more information on the possibilities of an additional study.

Below you can find a few of the very successful additional studies that have been or are being conducted within the Lifelines cohort. If you would like to know more about the studies below, or have a question regarding access to data and samples, please contact Lifelines.

Lifelines DEEP

The depth of phenotyping in the Lifelines DEEP cohort is unique and offers opportunities for groundbreaking research. The cohort comprises nearly 1500 Lifelines participants for whom we, besides the data and biological samples already collected in the regular Lifelines research program, now also have deep molecular phenotypes based on “omics” profiling, including: (1) whole-genome genotype data, (2) whole-genome methylation 450k data, (3) whole-genome gene expression data, (4) metabolomics data from plasma and exhaled breath, and (5) gut microbiome data. Lifelines DEEP has already resulted in 14 high-profile publications including several high-impact papers with wide-reaching results. In the April 2016 Science paper, for example, deep sequencing of the gut microbiome showed relationships between variations in the microbiome and 126 exogenous and intrinsic host factors. These results are an important step towards a better understanding of environment-diet-microbe-host interactions.

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Lifelines DAG3

Previously we collected additional data and biological samples in 1500 Lifelines participants to allow for new insights into disease mechanisms and prevention. In the subsequent study DAG3 we scaled up the number of participants as well as the number of microbiome sample sites. We enclosed  10,000 Lifelines participants and collected upon the regular collected biological samples:  PAXgene  blood, microbiome  samples from feces , throat, tongue and nose for among others sequencing  and culturing.  The “LifeLines DAG3” cohort is a truly unique cohort of extremely well phenotyped Dutch individuals that can help address many different scientific questions.

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Lifelines NEXT

In this observational study among Lifelines participants, 1500 mothers and their baby’s will be followed intensively. To investigate the effect of early life or pre-conceptional trans-generational events on healthy ageing and chronic disease in (early) childhood. Moreover, immunological, metabolic, environmental, (epi)genomic and social factors will be integrated with microbiome data to study early life development. This study includes the collection of biological samples: blood, urine, stool, placenta, breast milk, vaginal smear, nasal epithelial smear and buccal smear. This is combined with data collection on environmental factors via questionnaires.

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measurement of cortisol in scalp hair using LC-MS/MS: determination of a reference range and evaluation of hair sample collection in the Lifeines cohort study

The aim of this study is to create reference values for cortisol in scalp hair, obtained through a new measurement technique using LC-MS/MS. Furthermore, it is examined whether the associations between hair cortisol and waist circumference, BMI and metabolic parameters that were found using immunoassays can be confirmed using LC-MS/MS. Additionally, these results can be used to evaluate the protocol of hair collection in Lifelines in a pilot setting.

BIObanks Netherlands Internet Collaboration (BIONIC) - BBMRI-NL regenboogproject

This project aims to identify genetic loci related to depression, a common psychiatric disorder with often a chronic course. This proposal could help our understanding of the disease, and may help to target treatment.